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The Xiao Lab

We develop advanced single molecule imaging approahes in vivo and in vitro to study molecular and cellular mechanisms of cell division, cell wall synthesis, transcription regulation, and chromosome organization.

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Our Research

Recent News

The Xiao Lab is currently looking for a talented research technologist to join the team.

Click here for more information.

News

Chromosomal Topological Domain Formation Modulates Transcription and the Coupling of Neighboring Genes in Escherichia coli‘ by Drs. Nico Yehya, Christopher Bohrer, and collaborators is now available on bioRxiv. This extensive five-year study draws on the expertise of multiple labs and establishes chromosome topology as a key regulator of both transcription levels and the coupling between adjacent genes.

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Please enjoy Dr. Jie Xiao’s talk from the 2025 West Coast Superresolution Workshop on “Interrogating molecular organization and composition of the bacterial divisome using single-molecule and superresolution imaging”.

News

Selected Recent Publications

Lyu Z, Yang X, Yahashiri A, Ha S, McCausland J, Chen X, Britton B, Weiss D, Xiao J. Third track model for coordination of septal peptidoglycan synthesis and degradation by FtsN in Escherichia coli. Nature Microbiology. 2025 May. doi: 10.1038/s41564-025-02011-w.

 

McQuillen R, Perez AJ, Yang X, Bohrer CH, Smith EL, Chareyre S, Tsui HT, Bruce KE, Hla YM, McCausland JW, Winkler ME, Goley ED, Ramamurthi KS, Xiao J. Light-dependent modulation of protein localization and function in living bacteria cellsNat Commun. 2024 Dec 30;15(1):10746. doi: 10.1038/s41467-024-54974-9. PubMed PMID: 39737933; PubMed Central PMCID: PMC11685620.

 

Xinxing Yang, Ryan McQuillen, Zhixin Lyu, Polly Phillips-Mason, Ana De La Cruz, Joshua W McCausland, Hai Liang, Kristen E DeMeester, Cintia C Santiago, Catherine L Grimes, Piet de Boer, Jie XiaoA two-track model for the spatiotemporal coordination of bacterial septal cell wall synthesis revealed by single-molecule imaging of FtsW, Nature microbiology, 2021, 6(5), p584-593

 

CH Bohrer, X Yang, S Thakur, X Weng, B Tenner, R McQuillen, B Ross, Matthew Wooten, Xin Chen, Jin Zhang, Elijah Roberts, Melike Lakadamyali, Jie XiaoA pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM, Nature methods, 2021, 18 (6), 669-677

 

Weng X, Bohrer CH, Bettridge K, Lagda AC, Cagliero C, Jin DJ, Xiao JSpatial organization of RNA polymerase and its relationship with transcription in Escherichia coliProc Natl Acad Sci U S A. 2019 Sep 16;. doi: 10.1073/pnas.1903968116

 

Yang X, Lyu Z, Miguel A, McQuillen R, Huang KC, Xiao JGTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science. 2017 Feb 17;355(6326):744-747