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We are interested in taking single molecule measurements into living cells to study the organization, function, and dynamics of cellular processes.

Recent News

(June 2024) Dr. Amilcar Perez and Dr. Xiao‘s new review on “Stay on track — revelations of bacterial cell wall synthesis enzymes and things that go by single-molecule imaging” is now available in Curr. Opin Micro!

(May 2024) Dr. Jason Lyu‘s new manuscript on a “third sPG degradation track” FtsN coordinates septal peptidoglycan synthesis and degradation through self-interaction of SPOR in E. coli is now on bioRxiv!

(March 2024) Congrats to undergraduate student Boaz Goldberg for receiving PURA (Provost’s Undergraduate Research Award) for the upcoming summer research!

(February 2024) Congrats to graduate student Martin Yepes for receiving the Student Research Achievement Award from the 2024 Biophysical Society Annual Meeting!

(February 2024) Congrats to Dr. Brooke Britton and graduate student Martin Yepes for receiving the 2024 Biophysical Society Annual Meeting Travel Awards!

(February 2024) Dr. Xiao, Dr. Brooke Britton, and graduate students Fran Harris, Nico Yehya, and Martin Yepes attended the 2024 Biophysical Society Annual Meeting in Philadelphia and had fun!

(January 2024) Collaborative work between the Xiao lab and the Liu Lab on FtsZ-mediated spatial-temporal control over septal cell wall synthesis is now on BioRxiv!

(December 2023) “Protocol for single-molecule labeling and tracking of bacterial cell division proteins” is now available on STAR Protocols!

(December 2023)  “Differential roles of positive and negative supercoiling in organizing the E. coli genome” is now available on Nucleic Acids Research!

(November 2023) “Integration of cell wall synthesis and chromosome segregation during cell division in Caulobacter” is now available on J Cell Biol!

Select Publications

Zhixin Lyu, Atsushi Yahashiri, Xinxing Yang, Joshua W McCausland, Gabriela M Kaus, Ryan McQuillen, David S Weiss, Jie Xiao, FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli, Nature Communications, 2022, 13(1), p5751

Xinxing Yang, Ryan McQuillen, Zhixin Lyu, Polly Phillips-Mason, Ana De La Cruz, Joshua W McCausland, Hai Liang, Kristen E DeMeester, Cintia C Santiago, Catherine L Grimes, Piet de Boer, Jie Xiao, A two-track model for the spatiotemporal coordination of bacterial septal cell wall synthesis revealed by single-molecule imaging of FtsW, Nature microbiology, 2021, 6(5), p584-593

CH Bohrer, X Yang, S Thakur, X Weng, B Tenner, R McQuillen, B Ross, Matthew Wooten, Xin Chen, Jin Zhang, Elijah Roberts, Melike Lakadamyali, Jie Xiao, A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM, Nature methods, 2021, 18 (6), 669-677

Weng X, Bohrer CH, Bettridge K, Lagda AC, Cagliero C, Jin DJ, Xiao J. Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli. Proc Natl Acad Sci U S A. 2019 Sep 16;. doi: 10.1073/pnas.1903968116

Yang X, Lyu Z, Miguel A, McQuillen R, Huang KC, Xiao J. GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science. 2017 Feb 17;355(6326):744-747

Coltharp C, Buss J, Plumer TM, Xiao J. Defining the rate-limiting processes of bacterial cytokinesis. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1044-53