We are interested in taking single molecule measurements into living cells to study the organization, function, and dynamics of cellular processes.

Recent News

(August, 2021) Our preprint “FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in E. coli” is now available on BioRxiv!

(August, 2021) Dr. Gina Wang’s paper “Protein detection in blood with single-molecule imaging” has been published in Science Advances!

(June, 2021) Our paper “A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM” has been published in Nature Methods!

(June, 2021) An author profile on Dr. Jie Xiao been published in Nature Methods to accompany “A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM” !

(January, 2021) Our paper “Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism” has been published in Nature Communications!

(January, 2021) Our paper “A two-track model for the spatiotemporal coordination of bacterial septal cell wall synthesis revealed by single-molecule imaging of FtsW” has been published in Nature Microbiology!

(January, 2021) Post doc Dr. Amilcar Perez joins the lab. Welcome Dr. Perez!

(September, 2020) Dr. Christopher H. Bohrer’s chapter on the complexity and importance of diffusion in bacteria has been published in Physical Microbiology, Advances in Experimental Medicine and Biology. Congratulations Chris!

Select Publications

Weng X, Bohrer CH, Bettridge K, Lagda AC, Cagliero C, Jin DJ, Xiao J. Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli. Proc Natl Acad Sci U S A. 2019 Sep 16;. doi: 10.1073/pnas.1903968116

Yang X, Lyu Z, Miguel A, McQuillen R, Huang KC, Xiao J. GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science. 2017 Feb 17;355(6326):744-747

Coltharp C, Buss J, Plumer TM, Xiao J. Defining the rate-limiting processes of bacterial cytokinesis. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1044-53

Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J. A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics. PLoS Genet. 2015 Apr;11(4):e1005128

Hensel Z, Weng X, Lagda AC, Xiao J. Transcription-factor-mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cells. PLoS Biol. 2013;11(6):e1001591

Hensel Z, Feng H, Han B, Hatem C, Wang J, Xiao J. Stochastic expression dynamics of a transcription factor revealed by single-molecule noise analysis. Nat Struct Mol Biol. 2012 Aug;19(8):797-802