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The Xiao Lab

We are interested in taking single molecule measurements into living cells to study the organization, function, and dynamics of cellular processes.

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Our Research

Recent News

Dr. Xiao‘s editorial with Drs. Maria Filippa Addis and Ilana Kolodkin-Gal: “Women in microbial physiology and metabolism” is published in Front. Microbiol.! This editorial is part of the Research Topic “Women in Microbial Physiology and Metabolism: 2023″.

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Dr. Amilcar Perez and Dr. Xiao‘s new review on Stay on track — revelations of bacterial cell wall synthesis enzymes and things that go by single-molecule imaging” is now available in Curr. Opin Micro!

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Dr. Jason Lyu‘s new manuscript on a “third sPG degradation track” FtsN coordinates septal peptidoglycan synthesis and degradation through self-interaction of SPOR in E. coli is now on bioRxiv!

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Selected Recent Publications

Zhixin Lyu, Atsushi Yahashiri, Xinxing Yang, Joshua W McCausland, Gabriela M Kaus, Ryan McQuillen, David S Weiss, Jie XiaoFtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli, Nature Communications, 2022, 13(1), p5751

Xinxing Yang, Ryan McQuillen, Zhixin Lyu, Polly Phillips-Mason, Ana De La Cruz, Joshua W McCausland, Hai Liang, Kristen E DeMeester, Cintia C Santiago, Catherine L Grimes, Piet de Boer, Jie XiaoA two-track model for the spatiotemporal coordination of bacterial septal cell wall synthesis revealed by single-molecule imaging of FtsW, Nature microbiology, 2021, 6(5), p584-593

CH Bohrer, X Yang, S Thakur, X Weng, B Tenner, R McQuillen, B Ross, Matthew Wooten, Xin Chen, Jin Zhang, Elijah Roberts, Melike Lakadamyali, Jie XiaoA pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM, Nature methods, 2021, 18 (6), 669-677

Weng X, Bohrer CH, Bettridge K, Lagda AC, Cagliero C, Jin DJ, Xiao JSpatial organization of RNA polymerase and its relationship with transcription in Escherichia coliProc Natl Acad Sci U S A2019 Sep 16;doi: 10.1073/pnas.1903968116

Yang X, Lyu Z, Miguel A, McQuillen R, Huang KC, Xiao JGTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science2017 Feb 17;355(6326):744-747

Coltharp C, Buss J, Plumer TM, Xiao JDefining the rate-limiting processes of bacterial cytokinesis. Proc Natl Acad Sci U S A2016 Feb 23;113(8):E1044-53