Tool Development
Single-molecule localization based superresolution imaging has opened a wide door for biologists to explore the structures and dynamics of cellular assemblies at unprecedented resolutions. We develop computational methods and quantitative imaging analyses to counter blinking behaviors of fluorescent labels, correct confinement errors, and identify spatial features of cellular structures. Furthermore, we are developing new single-molecule imaging based assays to visualize the activity, localization and identity of protein factors and chromosomal DNA sequences.
Publications
Bohrer CH, Yang X, Weng X, Tenner B, Ross B, McQuillen R, Zhang J, Roberts E, Xiao J. A Pairwise Distance Distribution Correction (DDC) algorithm for blinking-free super-resolution microscopy. BioRxiv. 2019 September; doi: https://doi.org/10.1101/768051.
Mo GC, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J. Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution. Nat Methods. 2017 Apr;14(4):427-434.
Bohrer CH, Bettridge K, Xiao J. Reduction of Confinement Error in Single-Molecule Tracking in Live Bacterial Cells Using SPICER. Biophys J. 2017 Feb 28;112(4):568-574.
Xiao J, Dufrêne YF. Optical and force nanoscopy in microbiology. Nat Microbiol. 2016 Oct 26;1(11):16186. doi: 10.1038/nmicrobiol.2016.186.