We are interested in taking single molecule measurements into living cells to study the organization, function, and dynamics of cellular processes.

Recent News

(January, 2020) Post doc Dr. Amilcar Perez joins the lab. Welcome Dr. Britton!

(September, 2020) Dr. Christopher H. Bohrer’s chapter on the complexity and importance of diffusion in bacteria has been published in Physical Microbiology, Advances in Experimental Medicine and Biology. Congratulations Chris!

(July, 2020) Dr. Kelsey Bettridge’s paper on the role of nucleoid-associated protein, HU, in organizing the bacterial chromosome has been published in Molecular Microbiology. Congratulations Kelsey!

(June, 2020) First year PMB student Frances Harris joins the lab. Welcome Fran!

(June, 2020) Post doc Dr. Brooke Britton joins the lab. Welcome Dr. Britton!

(May, 2020) Congratulations to Ryan McQuillen for completing his PhD!

(Feb, 2020) Congratulations to Christopher H. Bohrer for completing his PhD!

(Dec, 2019) The whole is greater than the sum of its parts– check out how this philosophy of Aristotle is exemplified by the way of the nucleoid-associated protein HU organizing the bacterial chromosome by Dr. Kelsey Bettridge and our collaborators Drs. Subhash Verma and Sankar Adhya on BioRxiv.

(Nov, 2019) Bacterial cells do not have linear stepping motors, but they can still transport cargoes directionally. How? Find out from Josh McCausland and our collaborator Prof. Jian Liu‘s paper on BioRxiv.

(Nov, 2019) Dr. Xinxing Yang’s FtsW paper is now on BioRxiv! Check it out and see how smart E. coli cells use two tracks to organize their septal cell wall synthesis.

(Nov, 2019) Dr. Xinxing Yang gives a talk at the 6th Chesapeake Bay Area Single Molecule Biology meeting at University of Maryland.

(Sept, 2019) Ever dealt with fluorophore blinking in superresolution microscopy? It is worse than you think! Check out Chris Bohrer’s DDC algorithm to give you peace of mind. Now posted in BioRxiv.

Upcoming Events

Select Publications

Weng X, Bohrer CH, Bettridge K, Lagda AC, Cagliero C, Jin DJ, Xiao J. Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli. Proc Natl Acad Sci U S A. 2019 Sep 16;. doi: 10.1073/pnas.1903968116

Yang X, Lyu Z, Miguel A, McQuillen R, Huang KC, Xiao J. GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science. 2017 Feb 17;355(6326):744-747

Coltharp C, Buss J, Plumer TM, Xiao J. Defining the rate-limiting processes of bacterial cytokinesis. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1044-53

Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J. A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics. PLoS Genet. 2015 Apr;11(4):e1005128

Hensel Z, Weng X, Lagda AC, Xiao J. Transcription-factor-mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cells. PLoS Biol. 2013;11(6):e1001591

Hensel Z, Feng H, Han B, Hatem C, Wang J, Xiao J. Stochastic expression dynamics of a transcription factor revealed by single-molecule noise analysis. Nat Struct Mol Biol. 2012 Aug;19(8):797-802